Grouping of rice based on aromatic characteristic can be done by using the sense of smell, content analysis of compounds and molecular marker detection. One of the molecular marker technique for aromatic characteristic detection is Polymerase Chain Reaction (PCR). The objectives of this research were to detect an aromatic marker gene in nine upland rice lines (G9, G10, G12, G13, G19, G34, G35 and G136) and to check on whether the marker gene in homozygous or heterozygous state.

This research had been carried out from October to Desember 2009 at the Moleculer Biology Laboratory, Balai Besar BIOGEN, Bogor. Research procedures were as follows: (1) preparation of test materials of nine lines of upland rice seeds (G9, G10, G12, G13, G19, G34, G35, G39 and G136), the three parental of the cross (Mentik Wangi, Poso and Danau Tempe ), and three cultivars of comparation (Silugonggo, Situpatenggang and Pandan Wangi). Homozygous positive controls aromatic varieties (Mentik Wangi) and homozygous positive control of non-aromatic (Ciherang). (2) extraction of DNA from rice seeds based on a modified CTAB. (3) the process of PCR using four specific primers of aromatic rice marker gene (EAP, ESP, IFAP, INSP). (4) electrophoresis results of PCR on 1% agarose gel. (5) documentation of the DNA banding pattern of electrophoresis. (6) analyze results of  DNA  banding pattern from electrophoresis with descriptive analyze.

Results showed that eight lines (G9, G10, G13, G19, G34, G39 and G136) had the aromatic marker gene and one line (G35) had no aromatic marker gene. Mentik Wangi and Poso (parental cultivars) and Situpatenggang (cultivar of comparation) had the marker gene, while the aromatic cultivars Danau Tempe (parental cultivar) and Silugonggo (cultivar of comparation) had no aromatic marker gene. The aromatic gene in eight lines of aromatic  upland rice (G9, G10, G13, G19, G34, G39 and G136 was in the homozygous state.


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